研究業績

(#contributed equally)

2023
Mashima R, Takada S: Lysosomal acid lipase-deficiency: genetics, screening, animal models. International Journal of Molecular Sciences, in press

Mashima R, Ohira M, Okuyama T, Onodera M, Takada S. A novel mucopolysaccharidosis type II mouse model with an iduronate-2-sulfatase-P88L mutation. Scientific Reports. 13(1):7865 (2023).

Katoh-Fukui Y, Hattori A, Zhang R, Terao M, Takada S, Nakabayashi K, Hata K, Yamada Y, Matsuura N, Fukami M: Chromosomal microdeletion leading to pituitary gigantism through hormone-gene overexpression. Human Molecular Genetics. ddad053 (2023).

Yamada S, Ko T, Ito M, Sassa T, Nomura S, Okuma H, Sato M, Imasaki T, Kikkawa S, Zhang B, Yamada T, Seki Y, Fujita K, Katoh M, Kubota M, Hatsuse S, Katagiri M, Hayashi H, Hamano M, Takeda N, Morita H, Takada S, Toyoda M, Uchiyama M, Ikeuchi M, Toyooka K, Umezawa A, Yamanishi Y, Nitta R, *Aburatani H, Komuro I: TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. Science Advances. 9(15):eade7047 (2023).

Azuma N, Yokoi T, Tanaka T, Matsuzaka E, Saida Y, Nishina S, Terao M, Takada S, Fukami M, Okamura K, Maehara K, Yamasaki T, Hirayama J, Nishina H, Handa H, Yamaguchi Y: Integrator complex subunit 15 controls mRNA splicing and is critical for eye development. Human Molecular Genetics. ddad034 (2023).

Richard Albert J, Kobayashi T, Inoue A, Monteagudo-Sánchez A, Kumamoto S, Takashima T, Miura A, Oikawa M, Miura F, Takada S, Hirabayashi M, Korthauer K, Kurimoto K, Greenberg MVC, Lorincz M, Kobayashi H: Conservation and divergence of canonical and non-canonical imprinting in murids. Genome Biology. 24(1):48 (2023).

Ogawa Y, Terao M, Tsuji-Hosokawa A, Tsuchiya I, Hasegawa M, Takada S: SOX9 and SRY binding sites on mouse mXYSRa/Enh13 enhancer redundantly regulate Sox9 expression to varying degrees. Human Molecular Genetics, 32(1):55-64 (2023).

2022
Takeuchi I, Yanagi K, Takada S, Uchiyama T, Igarashi T, Motomura K, Hayashi Y, Nagano N, Matsuoka R, Sugiyama H, Yoshioka T, Saito H, Kawai T, Miyaji Y, Inuzuka Y, Matsubara Y, Ohya Y, Shimizu T, Matsumoto K, Arai K, Nomura I, Kaname T, Morita H: STAT6 gain-of-function variant exacerbates multiple allergic symptoms. The Journal of Allergy and Clinical Immunology, S0091-6749(22)02500-3 (2022).

Akiba K, Hasegawa Y, Katoh-Fukui Y, Terao M, Takada S, Hasegawa T, Fukami M, Narumi S: POU1F1/Pou1f1 c.143-83A>G variant disrupts the branch site in pre-mRNA and leads dwarfism. Endocrinology, 164(2):bqac198 (2022).

Terao M#, Ogawa Y#, Takada S, Kajitani R, Okuno M, Mochimaru Y, Matsuoka K, Itoh T, Toyoda A, Kono T, Jogahara T, Mizushima S, Kuroiwa A: Turnover of mammal sex chromosomes in the Sry-deficient Amami spiny rat is due to male-specific upregulation of Sox9, Proceedings of the National Academy of Sciences of the United States of America, 119(49):e2211574119 (2022).

Naiki Y, Miyado M, Shindo M, Horikawa R, Hasegawa Y, Katsumata N, Takada S, Akutsu H, Onodeara M, Fukami M: AAV-mediated gene therapy for patients' fibroblasts, iPS cells, and a mouse model of congenital adrenal hyperplasia. Human Gene Therapy, 33(15-16):801-809 (2022).

Nakagawa R, Takasawa K, Tsuji-Hosokawa A, Kawaji H, Murakawa Y, Takada S, Mikami M, Narumi S, Fukami M, Sreenivasan R, Maruyama T, Tucker E, Zhao L, Bowles J, Sinclair A, Koopman P, Hayashizaki Y, Morio T, Kashimada K: Two ovarian candidate enhancers, identified by time series enhancer RNA analyses, harbor rare genetic variations identified in ovarian insufficiency. Human Molecular Genetics, 31(13):2223-2235 (2022).

Tsuji-Hosokawa A, Ogawa Y, Tsuchiya I, Terao M, Takada S: Human SRY expression at the sex-determining period is insufficient to drive testis development in mice. Endocrinology, 163(1):bqab217 (2022).

Inoue M, Baba T, Takahashi F, Terao M, Miyabayashi K, Shima Y, Saito D, Sugiura K, Miura T, Takada S, Suyama M, Ohkawa Y, Morohashi K-I: Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway. Communications Biology, 5(1):974 (2022).

Mashima R, Takada S: Lipid nanoparticles: a novel gene delivery technique for clinical application. Current Issues in Molecular Biology, 44(10):5013-5027 (2022).

2021
Kajioka D, Suzuki K, Matsushita S, Hino S, Sato T, Takada S, Isono K, Takeo T, Kajimoto M, Nakagata N, Nakao M, Suyama M, DeFalco T, Miyagawa S, Yamada G. Sexual fate of murine external genitalia development: Conserved transcriptional competency for male-biased genes in both sexes. Proceedings of the National Academy of Sciences of the United States of America, 118(23):e2024067118 (2021).

Kagami M, Hara-Isono K, Matsubara K, Nakabayashi K, Narumi S, Fukami M, Ohkubo Y, Saitsu H, Takada S, Ogata T. ZNF445: a homozygous truncating variant in a patient with Temple syndrome and multilocus imprinting disturbance. Clinical Epigenetics, 13(1):119 (2021).

Hara S, Terao M, Tsuji-Hosokawa A, Ogawa Y, Takada S. Humanization of a tandem repeat in IG-DMR causes stochastic restoration of paternal imprinting at mouse Dlk1-Dio3 domain. Human Molecular Genetics, 30(7):564-574 (2021).

Otsuka K, Matsubara S, Shiraishi A, Takei N, Satoh Y, Terao M, Takada S, Kotani T, Satake H, Kimura AP. A testis-specific long noncoding RNA, Start, is a regulator of steroidogenesis in mouse Leydig cells. Frontiers in Endocrinology, section Experimental Endocrinology, 12:665874 (2021).

Miyamoto Y, Torii T, Terao M, Takada S, Tanoue A, Katoh H, Yamauchi J. Rnd2 differentially regulates oligodendrocyte myelination at different developmental periods. Molecular Biology of the Cell. 32(8):769-787 (2021).

Ushijima K#, Ogawa Y#, Terao M, Asakura Y, Muroya K, Hayashi M, Ishii T, Hasegawa T, Sekido R, Fukami M, Takada S, Narumi S.　Identification of the first promoter‐specific gain‐of‐function SOX9 missense variant (p.E50K) in a patient with 46,XX ovotesticular disorder of sex development. American Journal of Medical Genetics Part A, 185A:1067-1075 (2021).

Wakabayashi M, Tamura S, Kanzaki S, Kosugi M, Yoshimura Y, Ito T, Nagata K, Sato K, Takada S, Sekita Y, Kimura T. Five multicopy family genes expressed during maternal to zygote transition are not essential for mouse development. Biochemical and Biophysical Research Communications, 534:752-757 (2021).

2020
Akino R, Matsui D, Kawahara-Miki R, Amita M, Tatsumi K, Ishida E, Kang W, Takada S, Miyado K, Sekizawa A, Saito T, Kono T, Saito H. Next-generation sequencing reveals downregulation of the Wnt signaling pathway in human dysmature cumulus cells as a hallmark for evaluating oocyte quality. Reproductive Medicine, 1(3):205-215 (2020).

Tomikawa J, Takada S, Okamura K, Terao M, Ogata-Kawata H, Akutsu H, Tanaka S, Hata K, Nakabayashi K: Exploring trophoblast-specific Tead4 enhancers through chromatin conformation capture assays followed by functional screening. Nucleic Acids Research, 48(1):278-289 (2020).

Sato T, Kataoka K, Ito Y, Yokoyama S, Inui M, Mori M, Takahashi S, Akita K, Takada S, Ueno-Kudoh H, Asahara H. Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates. Elife, 9:e53608 (2020).

Akiba K, Narumi S, Nishimura R, Kato-Fukui Y, Takada S, Hasegawa Y, Fukami M. SOX9 is co-localized with paraspeckle protein NONO in cultured murine Sertoli cells and features structural characteristics of intrinsically disordered proteins. Molecular Reproduction and Development, 87(11):1124-1125 (2020).

Fujitani K, Otomo A, Nagayama Y, Tachibana T, Kato R, Kawashima Y, Kodera Y, Kato T, Takada S, Tamura K, Takamatsu N, Ito M: PACT/PRKRA and p53 regulate transcriptional activity of DMRT1. Genetics and Molecular Biology, 43(2):e20190017 (2020).

2019
Hara S, Muramatsu A, Terao M, Takada S: Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method. Scientific Reports, 9(1):20202 (2019).

Kagami M, Yanagisawa A, Ota M, Matsuoka K, Nakamura A, Matsubara K, Nakabayashi K, Takada S, Fukami M, Ogata T: Temple syndrome in a patient with variably methylated CpGs at the primary MEG3/DLK1:IG-DMR and severely hypomethylated CpGs at the secondary MEG3:TSS-DMR. Clinical Epigenetics 11(1) 42 (2019).

Miyado M#, Fukami M#, Takada S, Terao T, Nakabayashi K, Hata K, Matsubara Y, Tanaka Y, Sasaki G, Nagasaki K,Shiina M, Ogata K, Masunaga Y, Saitsu H, Ogata T: Nephrogenic syndrome of inappropriate antidiuresis caused by germline-derived gain-of-function mutations of GNAS Gsα-coding exons. Journal of the American Society of Nephrology, 30(5):877-889 (2019).

Maeoka Y, Okamoto, Wu Y, Saito A, Asada R, Matsuhisa K, Terao M, Takada S, Masaki T, Imaizumi K, Kaneko M: Renal medullary tonicity regulates RNF183 expression in the collecting ducts via NFAT5. Biochemical and Biophysical Research Communications, 25;514(2):436-442 (2019).

Sasaki K#, Shiba K#, Nakamura A, Kawano N, Satouh Y, Yamaguchi H, Morikawa M, Shibata D, Yanase R, Jokura J, Nomura M, Miyado M, Takada S, Ueno H, Nonaka S, Baba T, Ikawa M, Kikkawa M, Miyado K, Inaba1 K: Calaxin is required for cilia-driven determination of vertebrate laterality. Communications Biology, 2:226 (2019).

Yamoto K, Saitsu H, Nishimura G, Kosaki R, Takayama S, Haga N, Tonoki H, Okumura A, Horii E, Okamoto N, Suzumura H, Ikegawa S, Kato F, Fujisawa Y, Nagata E, Takada S, Fukami M, Ogata T: Comprehensive clinical and molecular studies in split-hand/foot malformation: identification of two plausible candidate genes (LRP6 and UBA2). European Journal of Human Genetics, 27(12):1845-1857 (2019).

Yamashita S#, Kataoka K#, Yamamoto H, Kato T, Hara S, Yamaguchi K, Renard-Guillet C, Katou Y, Shirahige K, Ochi H, Ogino H, Uchida T, Inui M, Takada S, Shigenobu S, Asahara H: Comparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken. Scientific Reports, 9(1):12560 (2019).

Sakai K, Ito C, Wakabayashi M, Kanzaki S, Ito T, Takada S, Toshimori K, Sekita Y, Kimura T: Usp26 mutation in mice leads to defective spermatogenesis depending on genetic background. Scientific Reports, 9(1):13757 (2019).

Shindo M, Inui M, Kang W, Tamano M, Tingwei C, Takada S, Hibino T, Yoshida M, Yoshida K, Okada H, Iwamoto T, Miyado K, Kawano N: Deletion of a seminal gene cluster reinforces a crucial role of SVS2 in male fertility. International Journal of Molecular Sciences, 20(18) pii:E4557 (2019).

2018
Ogawa Y#, Terao M#, Hara S#, Tamano M, Okayasu H, Kato T, Takada S: Mapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus. Scientific Reports, 8(1):17514 (2018).

Tsuji-Hosokawa A, Kashimada K, Kato T, Ogawa Y, Nomura R, Takasawa K, Lavery R, Coschiera A, Schlessinger D, Harley VR, Takada S, Morio T. Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular development. Scientific Reports, 8(1):13263 (2018).

Saito T#, Hara S#, Kato T, Tamano M, Muramatsu A, Asahara H, Takada S. A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Human Molecular Genetics, 27(18):3283-3292 (2018).

Inui M, Mokuda S, Sato T, Tamano M, Takada S, Asahara H: Dissecting the roles of miR-140 and its host gene. Nature Cell Biology, 20(5):516-518 (2018)

Hara S, Takada S: Genome editing for the reproduction and remedy of human diseases in mice. Journal of Human Genetics, 63(2):107-113 (2018).

Takasawa K, Gau M, Sutani A, Igarashi M, Ono M, Takemoto A, Takada S, Yamataka A, Ogata T, Morio T, Fukami M, Kashimada K: Phenotypic variation in 46,XX disorders of sex development due to the NR5A1 p.R92W variant: a sibling case report and literature review. Sexual Development, 11(5-6):284-288 (2018).

2017
Hara S, Terao M, Takada S: A Protocol for production of mutant mice using chemically synthesized crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Bio-protocol, 7(11):e2340 (2017).

Kajiwara K, Tanemoto T, Wada S, Karibe J, Ihara N, Ikemoto Y, Kawasaki T, Oishi Y, Samura O, Okamura K, Takada S, Akutsu H, Sago H, Okamoto A, Umezawa A: Fetal therapy model of myelomeningocele with three-dimensional skin using amniotic fluid cell-derived induced pluripotent stem cells. Stem Cell Reports, 8(6): 1701-171 (2017).

Kato T#, Hara S#, Goto Y, Ogawa Y, Okayasu H, Kubota S, Tamano M, Terao M, Takada S: Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system. Scientific Reports, 7:59 (2017).

Saito T#, Hara S#, Tamano M, Asahara H, Takada S: Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice. Journal of Reproduction and Development, 63:101-109.

Kato T, Takada S: In vivo and in vitro disease modeling with CRISPR/Cas9. Briefings in Functional Genomics, 16:13-24 (2017).

Inui M, Tamano M, Kato T, Takada S: CRISPR/Cas9-mediated simultaneous knockout of Dmrt1 and Dmrt3 does not recapitulate the 46,XY gonadal dysgenesis observed in 9p24.3 deletion patients. Biochemistry and Biophysics Reports, 9:238-244 (2017).

Igarashi M, Takasawa K, Hakoda A, Kanno J, Takada S, Miyado M, Baba T, Morohashi K, Tajima T, Hata K, Nakabayashi K, Matsubara Y, Sekido R, Ogata T, Kashimada K, Fukami M: Identical NR5A1 missense mutations in two unrelated 46,XX individuals with testicular tissues. Human Mutation, 38:39-42 (2017).

高田修治: ゲノム編集【難病研究up-to-date】. 遺伝子医学MOOK 32:245-250 (2017).

高田修治: ゲノム編集技術: ゲノム配列を自在に書き換える技術【COLUMN】. 周産期遺伝カウンセリング改訂2版 (Prenatal Genetic Counseling: A Practice Manual) pp181-183 (2017), 中外医学社

2016
Miyado M, Inui M, Igarashi M, Katoh-Fukui Y, Takasawa K, Hakoda A, Kanno J, Kashimada K, Miyado K, Tamano M, Ogata T, Takada S, Fukami M: The p.R92W variant of NR5A1/Nr5a1 induces testicular development of 46,XX gonads in humans, but not in mice: Phenotypic comparison of human patients and mutation-induced mice. Biology of Sex Differences, 7:56 (2016).

Akutsu H, Nasu M, Morinaga S, Motoyama T, Honma N, Machida M, Yamazaki-Inoue M, Okamura K, Nakabayashi K, Takada S, Nakamura N, Kanzaki S, Hata K, Umezawa A: In vivo maturation of human embryonic stem cell-derived teratoma over time. Regenerative Therapy, 5:31-39 (2016).

Hara S#, Kato T#, Goto Y, Kubota S, Tamano M, Terao M, Takada S: Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system. Journal of Reproduction and Development, 62(5): 531-536 (2016).
JRD Outstanding Paper Award 2016を受賞しました。

Okamura K, Sakaguchi H, Sakamoto-Abutani R, Nakanishi M, Nishimura K, Yamazaki-Inoue M, Ohtaka M, Periasamy VS, Alshatwi AA, Higuchi A, Hanaoka K, Nakabayashi K, Takada S, Hata K, Toyoda M, Umezawa A. Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA repair deficiency disorders. Scientific Reports, 6:26342 (2016).

Fujinaga H, Fujinaga H, Watanabe N, Kato T, Tamano M, Terao M, Takada S, Ito Y, Umezawa A, Kuroda M. Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia. American journal of Physiology. Lung cellular and molecular physiology, 310:L1143-1154 (2016).

Miyamoto Y, Tamano M, Torii T, Kawahara K, Nakamura K, Tanoue A, Takada S, Yamauchi J. Data supporting the role of Fyn in initiating myelination in the peripheral nervous system. Data in Brief, 1:1098-1105 (2016).

Terao M#, Tamano M#, Hara S#, Kato T, Kinoshita M, Takada S: Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas9. Experimental Animals, 65(3):275-283 (2016).

Tsumura H, Ito M, Takami M, Arai M, Li XK, Hamatani T, Igarashi A, Takada S, Miyado K, Umezawa A, Ito Y: Conditional deletion of CD98hc inhibits osteoclast development. Biochemistry and Biophysics Reports, 5:203-210 (2016).

2015
Miyamoto Y, Torii T, Takada S, Ohno N, Saitoh Y, Nakamura K, Ito A, Ogata T, Terada N, Tanoue A, Yamauchi J: Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination. Molecular Biology of the Cell, 26:3489-3503 (2015).

Katoh-Fukui Y#, Igarashi M#, Nagasaki K, Horikawa R, Nagai T, Tsuchiya T, Suzuki E, Miyado M, Hata K, Nakabayashi K, Hayashi K, Matsubara Y, Baba T, Morohashi K, Igarashi A, Ogata T, Takada S, Fukami M: Testicular dysgenesis/regression without campomelic dysplasia in patients carrying missense mutations and upstream deletion of SOX9. Molecular Genetics & Genomic Medicine, 3:550-557 (2015).

Hara S#, Tamano M#, Yamashita S, Kato T, Saito T, Sakuma T, Yamamoto T, Inui M, Takada S: Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Scientific Reports. 5:11221 (2015).

Matsubara Y#, Kato T#, Kashimada K, Tanaka H, Zhi Z, Ichinose S, Mizutani S, Morio T, Chiba T, Ito Y, Saga Y, Takada S, Asahara H: TALEN-mediated gene disruption on Y chromosome reveals critical role of EIF2S3Y in mouse spermatogenesis. Stem Cells and Development, 24:1164-1170 (2015).
表紙に採用されました

Miyata K, Miyata T, Nakabayashi K, Okamura K, Naito M, Kawai T, Takada S, Kato K, Miyamoto S, Hata K, Asahara H: DNA methylation analysis of human myoblasts during in vitro myogenic differentiation: de novo methylation of promoters of muscle-related genes and its involvement in transcriptional down-regulation. Human Molecular Genetics, 24:410-423 (2015).

Otabe K, Nakahara H, Hasegawa A, Matsukawa T, Ayabe F, Onizuka N, Inui M, Takada S, Ito Y, Sekiya I, Muneta T, Lotz M, Asahara H: Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo. Journal of Orthopaedic Research, 33:1-8 (2015).

2014
Onizuka N, Ito Y, Inagawa M, Nakahara H, Takada S, Lotz M, Toyama Y, Asahara H: The Mohawk homeobox transcription factor regulates the differentiation of tendons and volar plates. Journal of Orthopaedic Science 19:172-180 (2014).

Matsubara Y, Chiba T, Kashimada K, Morio T, Takada S, Mizutani S, Asahara H: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. Scientific Reports 4:5043 (2014).

Inui M, Miyado M, Igarashi M, Tamano M, Kubo A, Yamashita S, Asahara H, Fukami M, Takada S: Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Scientific Reports 4:5396 (2014).
Scientific Reportsのホームページ(Nature Japan)に注目の論文として掲載されました。

Fukawatase Y, Toyoda M, Okamura K, Nakamura K, Nakabayashi K, Takada S, Yamazaki-Inoue M, Masuda A, Nasu M, Hata K, Hanaoka K, Higuchi A, Takubo K, Umezawa A: Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability. Scientific Reports 4:5421 (2014).

Miyata K, Yotsumoto F, Nam SO, Odawara T, Manabe S, Ishikawa T, Itamochi H, Kigawa J, Takada S, Asahara H, Kuroki M, Miyamoto S: Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma. Cancer Medicine 3:1159-1169 (2014).

Nagata E, Kano H, Kato F, Yamaguchi R, Nakashima S, Takayama S, Kosaki R, Tonoki H, Mizuno S, Watanabe S, Yoshiura K, Kosho T, Hasegawa T, Kimizuka M, Suzuki A, Shimizu K, Ohashi H, Haga N, Numabe H, Horii E, Nagai T, Yoshihashi H, Nishimura G, Toda T, Takada S, Yokoyama S, Asahara H, Sano S, Fukami M, Ikegawa S, Ogata T:Japanese founder duplications/triplications involving BHLHA9 are associated with split-hand/foot malformation with or without long bone deficiency and Gollop-Wolfgang complex. Orphanet Journal of Rare Diseases 9:125 (2014).

Torii T, Miyamoto Y, Takada S, Tsumura H, Arai M, Nakamura K, Ohbuchi K, Yamamoto M, Tanoue A, Yamauchi J: In vivo knockdown of ErbB3 in miceinhibits Schwann cell precursor migration. Biochemical and Biophysical Research Communications, 452:782-788 (2014).

2013
Rakoczy J, Fernandez-Valverde SL, Glazov EA, Wainwright EN, Sato T, Takada S, Combes AN, Korbie DJ, Miller D, Grimmond SM, Little MH, Asahara H, Mattick JS, Taft RJ, Wilhelm D. MicroRNAs-140-5p/140-3p modulate Leydig cell numbers in the developing mouse testis. Biology of Reproduction, 88:143 (2013).

Shimizu H, Kubo A, Uchibe K, Hashimoto M, Yokoyama S, Takada S, Mitsuoka K, Asahara H. The AERO System: a 3D-like approach for recording gene expression patterns in the mouse whole embryo. PLoS ONE, 8:e75754 (2013).

Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Tamano M, Asahara H. Targeted gene deletion of miRNAs in mice by TALEN system. PLoS ONE, 8:e76004 (2013).

Kato T, Miyata K, Sonobe M, Yamashita S, Tamano M, Miyamoto S, Sakuma T, Yamamoto T, Inui M, Kikusui T, Asahara H, Takada S: Production of Sry knockout mouse using TALEN via oocyte injection. Scientific Reports, 3:3163 (2013).
BioTechniques誌のオンラインでこの論文が紹介されました。
日経バイオテク2013年12-2で紹介されました。
Scientific Reportsのホームページ(Nature Japan)に注目の論文として掲載されました。

2012
Fukami M#, Tsuchiya T#, Takada S#, Kanbara A, Asahara H, Igarashi A, Kamiyama Y, Nishimura G, Ogata T. Complex genomic rearrangement in the SOX9 5' region in a patient with Pierre Robin sequence and hypoplastic left scapula. American Journal of Medical Genetics Part A, 158A:1529-1534 (2012).

Yamashita S, Miyaki S, Kato Y, Yokoyama S, Sato T, Barrionuevo F, Akiyama H, Scherer G, Takada S, Asahara H. L-Sox5 and Sox6 enhance chondrogenic miR-140 expression by strengthening dimeric Sox9 activity. The Journal of Biological Chemistry, 287:22206-22215 (2012).

Takada S, Asahara H: Current strategies for microRNA research. Modern Rheumatology, 22:645-653 (2012).

高田修治: miRNAの合成経路. メディカル・サイエンス・ダイジェスト, 38: 98-101 (2012).

高田修治: マイクロRNAの網羅的発現解析. 医学のあゆみ, 241:159-160 (2012).

2011
高田修治: マイクロRNAの基礎と応用. 臨床整形外科, 46:418-423 (2011).

高田修治, 浅原弘嗣: 疾患に関するmRNAとmiRNAの検出法とその問題点. 臨床検査 55: 847-852 (2011).

2010
Hatanaka H, Takada S, Tsukui M, Choi YL, Kurashina K, Soda M, Yamashita Y, Haruta, H, Hamada T, Tamada, Tamada K, Hosoya Y, Sata N, Nagai H, Yasuda Y, Sugano K, Mano H Identification of transforming activity of Indian hedgehog by a retroviral expression screening. Cancer Science 101:60-64 (2010).

Yamashita Y, Yuan J, Suetake I, Suzuki H, Ishikawa Y, Choi Y-L, Ueno T, Soda M, Hamada T, Haruta H, Takada S, Miyazaki Y, Kiyoi H, Ito E, Naoe T, Tomonaga M, Toyota M, Tajima S, Iwama A, Mano H. Array-based genomic resequencing of human leukemia. Oncogene, 29:3723-3731 (2010).

Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz M, Ueno-Kudo H, Asahara H. microRNA-140 plays dual roles both in cartilage development and homeostasis. Genes & Development, 24:1173-1185 (2010).

2009
Kaneda R, Takada S, Yamashita Y, Choi YL, Nonaka-Sarukawa M, Soda M, Misawa Y, Isomura T, Shimada K, Mano H. Genome-wide histone methylation profile for heart failure. Genes to Cells 14:69-77 (2009).

Takeuchi K, Choi YL, Togashi Y, Soda M, Hatano S, Inamura K, Takada S, Ueno T, Yamashita Y, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y, Mano H. KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. Clinical Cancer Research 15:3143-3149 (2009).

Takada S, Berezikov E, Choi YL, Yamashita Y, Mano H. Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos. RNA 15:1507-1514 (2009).

Hatanaka H, Tsukui M, Takada S, Kurashina K, Choi YL, Soda M, Yamashita Y, Haruta H, Hamada T, Ueno T, Tamada K, Hosoya Y, Sata N, Yasuda Y, Nagai H, Sugano K, Mano H. Identification of transforming activity of free fatty acid receptor 2 by retroviral expression screening. Cancer Science 101:54-59 (2009).

Wada T, Yamashita Y, Saga Y, Takahashi K, Koinuma K, Choi YL, Kaneda R, Fujiwara S, Soda M, Watanabe H, Kurashina K, Hatanaka H, Enomoto M, Takada S, Mano H, Suzuki M. Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries. International Journal of Oncology 35:973-976 (2009).

2008
Takada S, Yamashita Y, Berezikov E, Hatanaka H, Fujiwara SI, Kurashina K, Watanabe H, Enomoto M, Soda M, Choi YL, Mano H. MicroRNA expression profiles of human leukemias. Leukemia 22:1274-1278 (2008).

O'Bryan MK, Takada S, Kennedy CL, Scott G, Harada S; Ray MK, Dai Q, Wilhelm D, de Kretser DM, Eddy EM, Koopman P, Mishina Y. Sox8 is a critical regulator of adult Sertoli cell function and male fertility. Developmental Biology 316:359-370 (2008).

Bray SJ, Takada S, Harrison E, Shen SC, Ferguson-Smith A: The atypical mammalian ligand Delta-like1 (Dlk1) can regulate Notch signalling in Drosophila. BMC Developmental Biology 8:11 (2008).

Choi YL, Takeuchi K, Soda M, Inamura K, Togashi Y, Hatano S, Enomoto M, Hamada T, Haruta H, Watanabe H, Kurashina K, Hatanaka H, Ueno T, Takada S, Yamashita Y, Sugiyama Y, Ishikawa Y and Mano H. Identification of novel isoforms of the EML4-ALK transforming gene in non-small cell lung cancer. Cancer Research 68:4971-4976 (2008).

Kurashina K, Yamashita Y, Ueno T, Koinuma K, Ohashi J, Horie H, Miyakura Y, Hamada T, Haruta H, Hatanaka H, Soda M, Choi YL, Takada S, Yasuda Y, Nagai H and Mano H. Chromosome copy number analysis in screening for prognosis-related genomic regions in colorectal carcinoma. Cancer Science 99:1835-1840 (2008).

Fujiwara SI, Yamashita Y, Nakamura N, Choi YL, Ueno T, Watanabe H, Kurashina K, Soda M, Enomoto M, Hatanaka H, Takada S, Abe M, Ozawa K and Mano H. High-resolution analysis of chromosome copy number alterations in angioimmunoblastic T-cell lymphoma and peripheral T-cell lymphoma, unspecified, with single nucleotide polymorphism-typing microarrays. Leukemia 22:1891-1898 (2008).

Takeuchi K, Choi YL, Soda M, Inamura K, Togashi Y, Hatano S, Enomoto M, Takada S, Yamashita Y, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y, Mano H. Multiplex Reverse Transcription-PCR Screening for EML4-ALK Fusion Transcripts. Clinical Cancer Research 14:6618-24 (2008).

Soda M, Takada S, Takeuchi K, Choi YL, Enomoto M, Ueno T, Haruta H, Hamada T, Yamashita Y, Ishikawa Y, Sugiyama Y, Mano H. A mouse model of EML4-ALK positive lung cancer. Proceedings of the National Academy of Sciences of the United States of America 105:19893-19897 (2008).

高田修治: レトロウィルスライブラリーを用いた癌関連マイクロRNAの同定, 自治医科大学医学部紀要 31:105 (2008).

2007
Fujiwara S, Yamashita Y, Choi YL, Watanabe H, Kurashina K, Soda M, Enomoto M, Hatanaka H, Takada S, Ozawa K, Mano H: Transforming activity of purinergic receptor P2Y, G protein coupled, 8 revealed by retroviral expression screening. Leukemia and Lymphoma 48:978-986 (2007).

Hatanaka H, Takada S, Choi YL, Fujiwara S, Soda M, Enomoto M, Kurashina K, Watanabe H, Yamashita Y, Sugano K, Mano: Transforming activity of purinergic receptor P2Y, G-protein coupled, 2 revealed by retroviral expression screening. Biochemical and Biophysical Research Communications 356:723-726 (2007).

Yamashita Y, Minoura K, Taya T, Fujiwara SI, Kurashina K, Watanabe H, Choi YL, Soda M, Hatanaka H, Enomoto M, Takada S, Mano H: Analysis of chromosome copy number in leukemic cells by different microarray platforms. Leukemia 21:1333-1337 (2007).

da Rocha ST, Tevendale M, Knowles E, Takada S, Watkins M, Ferguson-Smith AC: Restricted co-expression of Dlk1 and the reciprocally imprinted non-coding RNA, Gtl2: Implications for cis-acting control. Developmental Biology 306:810-823 (2007).

Choi YL, Kaneda R, Wada T, Fujiwara SI, Soda M, Watanabe H, Kurashina K, Hatanaka H, Enomoto M, Takada S, Yamashita Y, Mano H: Identification of a constitutively active mutant of JAK3 by retroviral expression screening. Leukemia Research 31:203-209 (2007).

Choi YL, Tsukasaki K, O'Neill MC, Yamada Y, Onimaru Y, Matsumoto K, Ohashi J, Yamashita Y, Tsutsumi S, Kaneda R, Takada S, Aburatani H, Kamihira S, Nakamura T, Tomonaga M, Mano H: A genomic analysis of adult T-cell leukemia. Oncogene 26:1245-1255 (2007).

Lin SP, Coan P, da Rocha ST, Seitz H, Cavaille J, Teng PW, Takada S, Ferguson-Smith AC: Differential regulation of imprinting in the murine embryo and placenta by the Dlk1-Dio3 imprinting control region. Development 134:417-426 (2007).

Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, Fujiwara S, Watanabe H, Kurashina K, Hatanaka H, Bando M, Ohno S, Ishikawa Y, Aburatani H, Niki T, Sohara Y, Sugiyama Y, Mano H. Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature 448:561-566 (2007).

Mano H, Takada S. mRAP, a sensitive method for determination of microRNA expression profiles. Methods 43:118-122 (2007).

Takada S and Mano H. Profiling of microRNA expression by mRAP. Nature Protocols 2:3136-3145 (2007).

2006
Koinuma K, Yamashita Y, Liu W, Hatanaka H, Kurashina K, Wada T, Takada S, Kaneda R, Choi YL, Fujiwara SI, Miyakura Y, Nagai H, Mano H: Epigenetic silencing of AXIN2 in colorectal carcinoma with microsatellite instability. Oncogene 25:139-146 (2006).

Takada S, Ota J, Kansaku N, Yamashita H, Izumi T, Ishikawa M, Wada T, Kaneda R, Choi YL, Koinuma K, Fujiwara SI, Aoki H, Kisanuki H, Yamashita Y, Mano H: Nucleotide sequence and embryonic expression of quail and duck Sox9 genes. General and Comparative Endocrinology 145:208-213 (2006).

Takada S, Wada T, Kaneda R, Choi YL, Yamashita Y, Mano H: Evidence for activation of Amh gene expression by steroidogenic factor 1. Mechanisms of Development 123, 472-480 (2006).

Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, Jan van Zonneveld A, Mano H, Plasterk R, Cuppen E: Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Research 16:1289-1298 (2006).

Takada S, Berezikov E, Yamashita Y, Lagos-Quintana M, Kloosterman WP, Enomoto M, Hatanaka H, Fujiwara SI, Watanabe H, Soda M, Choi YL, Plasterk RH, Cuppen E, Mano H: Mouse microRNA profiles determined with a new and sensitive cloning method. Nucleic Acids Research 34:e115 (2006).

Watanabe H, Yamashita Y, Takada S, Choi YL, Fujiwara S-i, Soda M, Enomoto M, Kurashina K, Hatanaka H, Kusama M, Mano H. Identification of activated RRAS2 in tongue carcinoma cell line. Oral Medicine & Pathology 11:121-126, (2006).

高田修治: 性決定の分子機構-比較ゲノム法とエピジェネティクス, Vision 11:17-19 (2006).

2005
Choi YL, Moriuchi R, Osawa M, Iwama A, Makishima H, Wada T, Kisanuki H, Kaneda R, Ota J, Koinuma K, Ishikawa M, Takada S, Yamashita Y, Oshimi K, Mano H: Retroviral expression screening of oncogenes in natural killer cell leukemia. Leukemia Research 29:943-949 (2005).

Kaneda R, Ueno S, Yamashita Y, Choi YL, Koinuma K, Takada S, Wada T, Shimada K, Mano H: Genome-wide screening for target regions of histone deacetylases in cardiomyocytes. Circulation Research 97:210-218 (2005).

Koinuma K, Kaneda R, Toyota M, Yamashita Y, Takada S, Choi YL, Wada T, Okada M, Konishi F, Nagai H, Mano H: Screening for genomic fragments that are methylated specifically in colorectal carcinoma with a methylated MLH1 promoter. Carcinogenesis 26:2078-2085 (2005).

Ishikawa M, Yoshida K, Yamashita Y, Ota J, Takada S, Kisanuki H, Koinuma K, Choi YL, Kaneda R, Iwao T, Tamada K, Sugano K, Mano H: Experimental trial for diagnosis of pancreatic ductal carcinoma based on gene expression profiles of pancreatic ductal cells. Cancer Science 96:387-393 (2005).

Kisanuki H, Choi YL, Wada T, Moriuchi R, Fujiwara SI, Kaneda R, Koinuma K, Ishikawa M, Takada S, Yamashita Y, Mano H: Retroviral expression screening of oncogenes in pancreatic ductal carcinoma. Europian Journal of Cancer 41:2170-2175 (2005).

Takada S, Mano H, Koopman P: Regulation of Amh during sex determination in chickens: Sox gene expression in male and female gonads. Cellular and Molecular Life Science 62:2140-2146 (2005).

Fujiwara S, Yamashita Y, Choi YL, Wada T, Kaneda R, Takada S, Maruyama Y, Ozawa K, Mano H: Transforming activity of the lymphotoxin-beta receptor revealed by expression screening. Biochemical and Biophysical Research Communications 338:1256-1262 (2005).

2004
Takada S, DiNapoli L, Capel B, Koopman P: Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles. Developmental Dymanics 231:387-395 (2004).

Kaneda R, Toyota M, Yamashita Y, Koinuma K, Choi YL, Ota J, Kisanuki H, Ishikawa M, Takada S, Shimada K, Mano H: High-throughput screening of genome fragments bound to differentially acetylated histones. Genes to Cells 9:1167-1174 (2004).

2003
Lin S-P, Youngson N, Takada S, Seitz H, Reik W, Paulsen M, Cavaille J, Ferguson-Smith AC: Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12. Nature Genetics 35:97-102 (2003).

Takada S, Koopman P. Origin and possible roles of the Sox8 transcription factor gene during sexual development. Cytogenetic and Genome Research 101:212-218 (2003).

2002
Takada S, Paulsen M, Tevendale M, Tsai C-E, Kelsey G, Cattanach BM, Ferguson-Smith AC: Epigenetic analysis of the Dlk1-Gtl2 imprinted domain on mouse chromosome 12: implications for imprinting control from comparison with Igf2-H19. Human Molecular Genetics 11:77-86 (2002).

Tsai C-E, Lin S-P, Ito M, Takagi N, Takada S, Ferguson-Smith AC: Genomic imprinting contributes to thyroid hormone metabolism in the mouse embryo. Current Biology 12:1221-1226 (2002).

2001
Paulsen M, Takada S, Youngson NA, Benchaib M, Charlier C, Segers K, Georges M, Ferguson-Smith AC: Comparative sequence analysis of the imprinted Dlk1 Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region. Genome Research 11:2085-2094 (2001).

2000
Kamiya M, Judson H, Okazaki Y, Kusakabe M, Muramatsu M, Takada S, Takagi N, Arima T, Wake N, Kamimura K, Satomura K, Hermann R, Bonthron DT, Hayashizaki Y: The cell cycle control gene ZAC/PLAGL1 is imprinted-a strong candidate gene for transient neonatal diabetes. Human Molecular Genetics 9:453-460 (2000).

Takada S, Tevendale M, Baker J, Georgiades P, Campbell E, Freeman T, Johnson MH, Paulsen M and Ferguson-Smith AC: Delta-like and Gtl2 are reciprocally expressed, differentially methylated linked imprinted genes on mouse chromosome 12. Current Biology 10:1135-1138 (2000).

1999
Takada S, Kamiya M, Arima T, Kagebayashi H, Shibata H, Muramatsu M, Chapman VM, Wake N, Hayashizaki Y, Takagi N: Detection and cloning of an X-Linked locus associated with a NotI site that is not methylated on mouse inactivated X chromosome by the RLGS-M method. Genomics 61:92-100 (1999).

1998
Ogura H, Takada S, Mise N, Sugimoto M, Tan S-S, Takagi N: Translocation breakpoint predisposes to X chromosome inactivation nonrandom in mouse embryos bearing Searle's T(X;16)16H translocation. Cytogenetics and Cell Genetics 80:173-178 (1998).

1997
Okuizumi H, Ohsumi T, Sasaki N, Imoto H, Mizuno Y, Hanami T, Yamashita H, Kamiya M, Takada S, Kitamura A, Muramatsu M, Nishumura M, Mori M, Matsuda Y, Tagaya O, Okazaki Y, Hayashizaki Y.: Linkage map of syrian Hamster with restriction landmark genomic scanning. Mammalian Genome 8:121-128 (1997).

1996
Takada S, Okazaki Y, Kamiya M, Ohsumi T, Nomura O, Okuizumi H, Sasaki N, Shibata H, Mori M, Nishimura M, Muramatsu M, Hayashizaki Y, Matsuda T: Five candidate genes for hamster cardiomyopathy did not map to the cardiomyopathylocus by FISH analysis. DNA Research 3:273-276 (1996).

Okazaki Y, Okuizumi H, Ohsumi T, Nomura O, Takada S, Kamiya M, Sasaki N, Matsuda Y, Nishimura M, Tagaya O, Muramatsu M, Hayashizaki Y: A genetic linkage map of the Syrian hamster and localization of cardiomyopathy locus on chromosome 9qa2.1-b1 using RLGS spot mapping. Nature Genetics 13:87-90 (1996).

Mise N, Sado T, Tada M, Takada S, Takagi N: Activation of the inactive X chromosome induced by cell fusion between a murine EC and female somatic cell accompanies reproducible change in the methylation pattern of the Xist gene. Experimental Cell Research 223:193-202 (1996).